Detection and Identification of Mycoplasma from Bovine Mastitis Infections Using a Nested Polymerase Chain Reaction

Detection Of Toxoplasma Gondii and Human Cytomegalovirus DNA in Blood from Transplant Recipients Using Multiplex Nested Polymerase Chain Reaction

Evidences from many studies suggested a polymerase chain reaction (PCR) as a valuable method for diagnosing infectious disease in the transplant recipients. We used this method for detection of Toxoplasma, gondii and human cytomegalovirus in blood specimens from patients after bone marrow or kidney transplantation. DNA of both infectious agents were detected using two separate sets of nested pr...

Isolation and identification of Mycoplasma agalactiae by culture and polymerase chain reaction (PCR) from sheep of Qom province, Iran

Contagious agalactia (C.A) is an infectious syndrome of sheep that is characterized by mastitis and subsequent failure of milk production, arthritis, abortion and keratoconjunctivitis. Mycoplasma agalactiae (M. agalactiae) is the main cause of the disease in sheep. The aim of this study was isolation and identification of M. agalactiae with culture and polymerase chain reaction (PCR) assay from...

Polymerase Chain Reaction of Mgc2 and 16S rRNA Genes for Detection of Mycoplasma gallisepticum

Mycoplasmas are very small bacteria lacking cell walls that belong to various genera within the class Mollicutes, and also the smallest organisms that can live independently. They are able to cause serious and chronic disease because of some unique characteristics. Mycoplasma gallisepticum (MG) is an important avian pathogen causing significant economical losses within the poultry industry. The...

Detection of Babesia ovis Using Polymerase Chain Reaction.

Detection of Mycoplasma pneumoniae by using the polymerase chain reaction.

The polymerase chain reaction (PCR) technique was used to detect Mycoplasma pneumoniae. A specific DNA sequence for M. pneumoniae was selected from a genomic library, and two oligonucleotides were chosen in this sequence to give an amplified fragment of 144 base pairs. We show that DNA from different M. pneumoniae strains can be detected by PCR, with DNA from other Mycoplasma species giving neg...